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Adiponectin inhibits oxidization-induced differentiation of T helper cells through inhibiting costimulatory CD40 and CD80 BJMBR
Xiao,Y.; Deng,T.; Shang,Z.; Wang,D..
Adiponectin is a multifunctional adipokine that has several oligomeric forms in the blood stream, which broadly regulates innate and acquired immunity. Therefore, in this study, we aimed to observe the differentiation of T helper (Th) cells and expression of costimulatory signaling molecules affected by adiponectin. The mRNA and protein expression levels of adiponectin and its receptors in oxidized low density lipoprotein cholesterol-treated endothelial cells were assayed by real time PCR and immunofluorescence. The endothelial cells were then treated with adiponectin with or without adipoR1 or adipoR2 siRNA and co-cultured with T lymphocytes. The distribution of Th1, Th2 and Th17 subsets were assayed by flow cytometry. The effects of adiponectin on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adiponectin; T helper cells; HLA-DR; CD80; CD40.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600606
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Efecto del virus del síndrome reproductivo y respiratorio porcino (PRRS) en células dendríticas de cerdo derivadas de monocitos Veterinaria México
Flores-Mendoza,Lilian; Silva-Campa,Erika; Reséndiz,Mónica; Mata-Haro,Verónica; Osorio,Fernando A.; Hernández,Jesús.
Las células dendríticas (DC) son las presentadoras de antígeno más importantes del sistema inmune. Su localización anatómica (piel, mucosas y sangre periférica), la expresión de receptores para reconocer patógenos, la expresión de moléculas de coestimulación (CD80/86), del complejo principal de histocompatibilidad (MHC) clases I y II, y la producción de citocinas (IFN-α, IL-10, IL-12), les confiere una característica única para regular las respuestas inmune innata y adaptativa. El objetivo de este trabajo fue evaluar el efecto del virus de síndrome reproductivo y respiratorio porcino (PRRS) en DC maduras. Se generaron células dendríticas a partir de monocitos utilizando IL-4 y GM-CSF y se estimularon con lipopolisacárido (LPS) para inducir su maduración....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Células dendríticas; Cerdo; CD80; CD86; MHC-II; RT-PCR.
Ano: 2009 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922009000100005
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Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates BABT
Oliveira,Flávia Márcia; Ruiz-de-Souza,Valéria; Campana-Pereira,Maria Aparecida; Gontijo,Cristiano Machado.
One of the mechanisms by which adjuvants are believed to promote T-cell activation and prevent induction of oral tolerance is by up-regulating the expression of co-stimulatory molecules on antigen presenting cells. Mice treated orally with palmitoyl-ovalbumin conjugates become immunized, while those treated with native ovalbumin (Ova) become tolerant. Cells from the peritoneal cavity of B6D2F1 mice were cultured in the presence of 0.01, or 0.1 mg/100ml of either Ova, or palmitoyl-Ova and tested for the presence of cell markers. PE-conjugated anti-mouse CD80, CD86, and CD11b antibodies as well as biotin-PE were used to stain the antigen-activated peritoneal cells. A significant increase in the expression of CD86 and CD80 was observed following in vitro...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovalbumin; Palmitic Acid; Cellular Immunity; CD86; CD80; CD11b.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600011
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